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9:30 - 10:45 |
11:15 - 12:30 |
13:00 - 14:00 |
14:00 - 17:30 |
18:00 - 19:00 |
19:15 - 21:00 |
5 Tues |
Arrival |
Overview of the course, Introductions Lecture 1 - EM Preparation Methods Carolyn Moores |
Dinner |
Guest Lecture 1 Paula da Fonseca |
6 Wed |
Lecture 2 Basic concepts of Fourier analysis
Giulia Zanetti |
Lecture 3 Concepts of convolution, point spread, and cross-correlation functions
Elena Orlova |
Lunch |
Practical 1
Concepts of Fourier Tranform, EMAN 2
Instructors: H.Saibil
Instructions and Data Files Zip File .tar.gz
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Dinner |
Guest Lecture 2 Patrick Cramer |
7 Thurs |
Lecture 4 Principles of TEM image formation
Peter Rosenthal |
Lecture 5 Contrast Transfer & CTF correction
Helen Saibil |
Lunch |
Practical 2 Scipion: Frame alignment, Contrast transfer & CTF Correction
(Movie corrections, ctffind4, CTF Correction)
Instructors: Carlos Oscar Sorzano & Jose Miguel de la Rosa Trevin
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Dinner |
Poster Session 1
Group 1 |
8 Fri |
Lecture 6 Image acquisition, noise, frame alignment, particle detection
Helen Saibil |
Lecture 7 MSA & classification of single particles
Dan Clare |
Lunch |
Lecture 8 DE Detectors Ben Bammes
Lecture 8a Gatan Paul Mooney
Practical 3 Scipion: Statistical Analysis and MSA, Eigen Vectors
Instructors: Carlos Oscar Sorzano & Jose Miguel de la Rosa Trevin
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Dinner |
Guest lecture 3
Werner Kuelbrandt |
9 Sat |
Lecture 9 Point Group Symmetries
Elena Orlova |
Lecture 10 Reconstruction principles and Tomography strategy
Juha Huiskonen
12:30 Molecular Dimensions Claire Naylor
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Lunch |
Practical 4 Tomography and sub-tomogram averaging
Instructors: Natasha Lukoyanova
Data Files Zip File .tar.gz
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Dinner |
Poster Session Selected student talks
Group 1 |
10 Sun |
Free Day |
Group excursion London Eye at 5:00 pm |
Course dinner at 18:30 pm |
11 Mon |
Lecture 11 Methods for determination of angular orientations
Elena Orlova |
Lecture 12 Methods of 3D EM reconstructions, assessment of resolution
Sarah Butcher |
Lunch |
13:45 Lecture 13 A Baysesian approach in analysis of cryo-images Sjors Scheres
Practical 5 Relion
Instructors: Guilia Zanetti & Sjors Scheres
Data Files Relion Tutorial Data
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Dinner |
Guest Lecture 4 Richard Henderson |
12 Tues |
Lecture 14 CCPEM
Tom Burnley |
Lecture 15 Reconstruction of viruses
Michael Rossmann |
Lunch |
13:45 Lecture 16 JEOL - Andy Yarwood
Practical 6 Relion
Instructors: Guilia Zanetti & Sjors Scheres
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Dinner |
Guest lecture 5
Radostin Danev |
13 Weds |
Lecture 17 Vaildation of 3D EM reconstructions
Peter Rosenthal |
lecture 18 Interpretation of maps, docking of atomic structure
Agnel Praveen Joseph |
Lunch |
Practical 7 Fitting of stuctures, flexible fitting
Instructors: Agnel Praveen Joseph
Data Files Zip File .tar.gz
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Dinner |
Poster Session
Group 2 |
14 Thurs |
Lecture 19 Microscopes for cryo - software for automated data collection
Felix de Haas |
Lecture 20 Refmac
Garib Murshudov |
Lunch |
Practical 8 Fitting COOT
Coot. Instructors: Paul Emsley
Data Files Zip File .tar.gz
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Dinner |
Poster Session Selected student talks Group 2
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15 Fri |
Lecture 21 EMDB Data deposition
Ardan Patwardhan |
Guest Lecture 6
Gabriel Waksman |
Lunch |
Course Review and departure |
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